Controls of the expression of aspA, the aspartyl protease gene from Penicillium roqueforti

  title={Controls of the expression of aspA, the aspartyl protease gene from Penicillium roqueforti},
  author={St{\'e}phanie Gente and N. Durand-Poussereau and Michel Fěvre},
  journal={Molecular and General Genetics MGG},
The gene (aspA) encoding the extracellular aspartyl protease from Penicillium roqueforti was cloned and characterized. Northern hybridization analyses and β-casein degradation assays revealed that aspA was strongly induced by casein in the medium and efficiently repressed by ammonia. External alkaline pH overrides casein induction, resulting in aspA repression. Cis-acting motifs known to mediate nitrogen and pH regulation of fungal gene expression are present in the aspA promoter and protein… CONTINUE READING


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