Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.

@article{Blazeck2012ControllingPS,
  title={Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters.},
  author={John J. Blazeck and Rishi Garg and Ben Reed and Hal S. Alper},
  journal={Biotechnology and bioengineering},
  year={2012},
  volume={109 11},
  pages={2884-95}
}
A dynamic range of well-controlled constitutive and tunable promoters are essential for metabolic engineering and synthetic biology applications in all host organisms. Here, we apply a synthetic hybrid promoter approach for the creation of strong promoter libraries in the model yeast, Saccharomyces cerevisiae. Synthetic hybrid promoters are composed of two modular components-the enhancer element, consisting of tandem repeats or combinations of upstream activation sequences (UAS), and the core… CONTINUE READING
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