Controlled proteolysis of nascent polypeptides in rat liver cell fractions. II. Location of the polypeptides in rough microsomes.

@article{Sabatini1970ControlledPO,
  title={Controlled proteolysis of nascent polypeptides in rat liver cell fractions. II. Location of the polypeptides in rough microsomes.},
  author={David D. Sabatini and Gunter Blobel},
  journal={The Journal of cell biology},
  year={1970},
  volume={45 1},
  pages={146-57}
}
Rough microsomes were incubated in an in vitro amino acid-incorporating system for labeling the nascent polypeptide chains on the membrane-bound ribosomes. Sucrose density gradient analysis showed that ribosomes did not detach from the membranes during incorporation in vitro. Trypsin and chymotrypsin treatment of microsomes at 0 degrees led to the detachment of ribosomes from the membranes; furthermore, trypsin produced the dissociation of released, messenger RNA-free ribosomes into subunits… CONTINUE READING

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References

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Showing 1-10 of 12 references

Studies on the formation of transfer ribonucleic acidribosome complexes

  • R. E. MONRO
  • IV . J . Biol . Chem .
  • 1968

The synthesis of serum proteins on attached rather than free ribosomes of rat liver

  • REDMAN, M C.
  • Biochem . Biophys. Res
  • 1968

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