Controlled layer-by-layer immobilization of horseradish peroxidase.

@article{Rao1999ControlledLI,
  title={Controlled layer-by-layer immobilization of horseradish peroxidase.},
  author={S. Venkateswara Rao and Kevin W Anderson and Leonidas G Bachas},
  journal={Biotechnology and bioengineering},
  year={1999},
  volume={65 4},
  pages={389-96}
}
Horseradish peroxidase (HRP) was biotinylated with biotinamidocaproate N-hydroxysuccinimide ester (BcapNHS) in a controlled manner to obtain biotinylated horseradish peroxidase (Bcap-HRP) with two biotin moieties per enzyme molecule. Avidin-mediated immobilization of HRP was achieved by first coupling avidin on carboxy-derivatized polystyrene beads using a carbodiimide, followed by the attachment of the disubstituted biotinylated horseradish peroxidase from one of the two biotin moieties… CONTINUE READING

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