Controlled insertional mutagenesis using a LINE-1 (ORFeus) gene-trap mouse model.

Abstract

A codon-optimized mouse LINE-1 element, ORFeus, exhibits dramatically higher retrotransposition frequencies compared with its native long interspersed element 1 counterpart. To establish a retrotransposon-mediated mouse model with regulatable and potent mutagenic capabilities, we generated a tetracycline (tet)-regulated ORFeus element harboring a gene-trap… (More)
DOI: 10.1073/pnas.1302504110

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