Controlled Expression of Recombinant Proteins in Physcomitrella patens by a Conditional Heat-shock Promoter: a Tool for Plant Research and Biotechnology

@article{Saidi2005ControlledEO,
  title={Controlled Expression of Recombinant Proteins in Physcomitrella patens by a Conditional Heat-shock Promoter: a Tool for Plant Research and Biotechnology},
  author={Younousse Saidi and Andrija Finka and Mickhail Chakhporanian and Jean-Pierre Zr{\"y}d and Didier G. Schaefer and Pierre Goloubinoff},
  journal={Plant Molecular Biology},
  year={2005},
  volume={59},
  pages={697-711}
}
The ability to express tightly controlled amounts of endogenous and recombinant proteins in plant cells is an essential tool for research and biotechnology. Here, the inducibility of the soybean heat-shock Gmhsp17.3B promoter was addressed in the moss Physcomitrella patens, using β-glucuronidase (GUS) and an F-actin marker (GFP-talin) as reporter proteins. In stably transformed moss lines, Gmhsp17.3B-driven GUS expression was extremely low at 25 °C. In contrast, a short non-damaging heat… CONTINUE READING