Control of synthesis of wall teichoic acid during balanced growth of Bacillus subtilis W23.

Abstract

Enzymes involved in the synthesis of teichoic acid and its linkage to the wall in Bacillus subtilis W23 were measured in chemostat cultures growing at equilibrium at a dilution rate of 0.2 h-1 in different concentrations of inorganic phosphate. All the enzymes, except teichoic acid glucosyl transferase, which was insensitive to changes in phosphate concentration, were almost undetectable at 0.5 mM-phosphate. At higher phosphate concentrations the changes in activity of the enzymes of linkage unit synthesis were sufficient to account for the changes in the rate of incorporation of teichoic acid into the wall in vivo. Between 3.5 and 4.5 mM-phosphate the amount of teichoic acid synthesized in vivo increased, but no increase in the ability of toluenized bacteria to synthesize teichoic acid could be detected. Allosteric regulation might therefore be important at high phosphate concentrations. Bacteria maintained a constant ATP content and a constant adenylate energy charge during chemostat growth at all phosphate concentrations.

Statistics

050100150'85'88'91'94'97'00'03'06'09'12'15
Citations per Year

62 Citations

Semantic Scholar estimates that this publication has 62 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Cheah1982ControlOS, title={Control of synthesis of wall teichoic acid during balanced growth of Bacillus subtilis W23.}, author={Suan Choo Cheah and Heather M Hussey and Ian C. Hancock and James Baddiley}, journal={Journal of general microbiology}, year={1982}, volume={128 3}, pages={593-9} }