Control of rabbit liver fructose-1, 6-diphosphatase activity by magnesium ions.

@article{Tashima1975ControlOR,
  title={Control of rabbit liver fructose-1, 6-diphosphatase activity by magnesium ions.},
  author={Yohtalou Tashima and Natsue Yoshimura},
  journal={Journal of biochemistry},
  year={1975},
  volume={78 6},
  pages={1161-9}
}
EDTA at a concentration of 1 muM produced a threshold effect in the activation of purified rabbit liver fructose-1, 6-diphosphatase [EC 3.1.3.11] in the presence of 5 mM Mg2+ at pH 7.2. Without EDTA, biphasic activation curves were produced by Mg2+. A double-reciprocal plot of the data gave the Km values corresponding to the two linear regions. They were 0.19 and 0.83 mM at pH 7.5, and 0.055 and 0.83 mM at pH 9.1. In the presence of 5muM EDTA a sigmoidal curve was obtained for Mg2+ activation… CONTINUE READING