Control of pollen-mediated gene flow in transgenic trees.

  title={Control of pollen-mediated gene flow in transgenic trees.},
  author={Chunsheng Zhang and Kim H. Norris-Caneda and William H. Rottmann and Jon E. Gulledge and Shujun Chang and Brian Kwan and Anita M. Thomas and Lydia C. Mandel and Ronald T. Kothera and Aditi D. Victor and Leslie Pearson and Maud A. W. Hinchee},
  journal={Plant physiology},
  volume={159 4},
Pollen elimination provides an effective containment method to reduce direct gene flow from transgenic trees to their wild relatives. Until now, only limited success has been achieved in controlling pollen production in trees. A pine (Pinus radiata) male cone-specific promoter, PrMC2, was used to drive modified barnase coding sequences (barnaseH102E, barnaseK27A, and barnaseE73G) in order to determine their effectiveness in pollen ablation. The expression cassette PrMC2-barnaseH102E was found… CONTINUE READING
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