Control of directionality in the site-specific recombination system of the Streptomyces phage phiC31.

@article{Thorpe2000ControlOD,
  title={Control of directionality in the site-specific recombination system of the Streptomyces phage phiC31.},
  author={Helena M Thorpe and scott. wilson and Margaret C. M. Smith},
  journal={Molecular microbiology},
  year={2000},
  volume={38 2},
  pages={232-41}
}
The genome of the Streptomyces temperate phage phiC31 integrates into the host chromosome via a recombinase belonging to a novel group of phage integrases related to the resolvase/invertase enzymes. Previously, it was demonstrated that, in an in vitro recombination assay, phiC31 integrase catalyses integration (attP/attB recombination) but not excision (attL/attR). The mechanism responsible for this recombination site selectivity was therefore investigated. Purified integrase was shown to bind… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 81 extracted citations

Aminocoumarins mutasynthesis, chemoenzymatic synthesis, and metabolic engineering.

Methods in enzymology • 2009
View 3 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…