Control of demand-driven biosynthesis of glutathione in green Arabidopsis suspension culture cells.

@article{Meyer2002ControlOD,
  title={Control of demand-driven biosynthesis of glutathione in green Arabidopsis suspension culture cells.},
  author={Andreas J Meyer and Mark D. Fricker},
  journal={Plant physiology},
  year={2002},
  volume={130 4},
  pages={1927-37}
}
We have investigated what limits demand-driven de novo glutathione (GSH) biosynthesis in green Arabidopsis suspension culture cells. GSH is the most abundant low-molecular weight thiol in most plants and can be quantified using monochlorobimane to fluorescently label GSH in live cells. Progress curves for labeling reached a plateau as all the cytoplasmic GSH was conjugated. In the presence of excess monochlorobimane, a second, almost linear phase of labeling was observed, after a lag of 2 to 3… CONTINUE READING

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