Control of crystal phase switching and orientation by soluble mollusc-shell proteins

  title={Control of crystal phase switching and orientation by soluble mollusc-shell proteins},
  author={Angela M. Belcher and X. H. Wu and Renee. J. Christensen and Paul K. Hansma and Galen D. Stucky and D. E. Morse},
IN the initial stages of the biomineralization of abalone shells, a primer layer of oriented calcite crystals grows on a nucleating protein sheet1,2. The deposition of this primer is followed by an abrupt transition to c-axis-oriented crystals of aragonite, another crystalline form of calcium carbonate. The formation of each of the two crystal types is accompanied by the synthesis of specific polyanionic proteins1–3, suggesting that cooperative interactions between these proteins and the… 
Structural changes in a protein fragment from abalone shell during the precipitation of calcium carbonate.
This report represents one of the first studies on the conformational changes of a protein fragment that is involved in biomineralization processes on moving from the solution phase into the mineral phase and indicates that GP controls the precipitation kinetics and morphology of calcium carbonate crystals.
Characterization of two molluscan crystal‐modulating biomineralization proteins and identification of putative mineral binding domains
Two proteins isolated from the aragonitic component of nacre of the red abalone, Haliotis rufescens, are characterized, demonstrating that protein fractions enriched in AP7 and AP24 produced CaCO3 crystals with morphology distinct from crystals grown in the presence of the total mixture of soluble aragonite‐specific proteins.
Sequential switch of biomineral crystal morphology using trivalent ions
A new strategy by which sequential addition or depletion of inorganic trivalent ions in a supersaturated solution can be used to switch the surface morphology of calcium oxalate monohydrate (COM) back and forth, resulting in either the growth of flat crystalline sheets or of nanostructures oriented perpendicular to the surface.
Amorphous-to-crystal transition in the layer-by-layer growth of bivalve shell prisms
Biomineralization integrates complex physical and chemical processes bio-controlled by the living organ- isms through ionic concentration regulation and organic molecules production. It allows tuning
Patterns of Expression in the Matrix Proteins Responsible for Nucleation and Growth of Aragonite Crystals in Flat Pearls of Pinctada fucata
The initial growth of the nacreous layer is crucial for comprehending the formation of nacreous aragonite. A flat pearl method in the presence of the inner-shell film was conducted to evaluate the
Morphologies and Growth Model of Biomimetic Fabricated Calcite Crystals Using Amino Acids and Insoluble Matrix Membranes of Mytilus edulis
Protein membranes extracted from mollusk shells were selected as substrates to precipitate calcium carbonate crystals in CaCl2 solutions containing glycine or aspartic acid. This research mainly
“Liquid-like” biomineralization protein assemblies: a key to the regulation of non-classical nucleation
Over the past several decades, numerous studies have attempted to define the general role that proteins play in the nucleation of biominerals in organisms. Recently, with the emergence of the
Direct observation of the transition from calcite to aragonite growth as induced by abalone shell proteins.


Genetic Coding in Biomineralization of Microlaminate Composites
Biomineralization is precisely controlled by complex templating relationships ultimately encoded in the genes. In the formation of the molluscan shell, polyanionic pleated sheet proteins serve as
Critical Transitions in the Biofabrication of Abalone Shells and Flat Pearls
Analyses of biopolymer/calcium carbonate composites grown on inorganic abiotic substrates implanted between the shell and the shell-secreting epithelium of live red abalones (Haliotis rufescens)
Morphogenesis of calcitic sponge spicules: a role for specialized proteins interacting with growing crystals.
  • J. Aizenberg, J. Hanson, S. Weiner
  • Materials Science
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 1995
It is concluded that highly controlled intercalation of specialized proteins inside the crystals is an additional means by which organisms control spicule growth.
Organization of extracellularly mineralized tissues: a comparative study of biological crystal growth.
  • S. Weiner
  • Materials Science
    CRC critical reviews in biochemistry
  • 1986
The study shows that there is a common basis for understanding these mineralization processes which is reflected in the nature of the protein-crystal interactions which occur in each tissue.
Crystallization at Inorganic-organic Interfaces: Biominerals and Biomimetic Synthesis
A biomimetic approach based on these principles could lead to the development of new strategies in the controlled synthesis of inorganic nanophases, the crystal engineering of bulk solids, and the assembly of organized composite and ceramic materials.
Flat pearls from biofabrication of organized composites on inorganic substrates
THE study of biomineralization is inspiring new approaches to the controlled fabrication of synthetic materials such as nanoparticles, polymer–mineral composites and templated crystals1–3. Although
Control of Aragonite or Calcite Polymorphism by Mollusk Shell Macromolecules
Many mineralizing organisms selectively form either calcite or aragonite, two polymorphs of calcium carbonate with very similar crystalline structures. Understanding how these organisms achieve this
In investigating the influence of the matrix, the crystal types which form on decalcified matrices taken from various molluscan species and introduced into other species or recalcified in vitro are determined.
Biomineralization: Chemical and Biochemical Perspectives
The functional forms of biominerals, R.J.P.Williams crystallochemical strategies in biomineralization, S.Mann carbonate calcification in algae - initiation and control, M.A.Borowitzka matrix-crystal