Control of O-Glycan Branch Formation

  title={Control of O-Glycan Branch Formation},
  author={Tilo Schwientek and Mitsuharu Nomoto and Steven B. Levery and Gerard F. M. Merkx and Ad Geurts van Kessel and Eric Paul Bennett and Michael A. Hollingsworth and Henrik Clausen},
  journal={The Journal of Biological Chemistry},
  pages={4504 - 4512}
A novel human UDP-GlcNAc:Gal/GlcNAcβ1–3GalNAcα β1,6GlcNAc-transferase, designated C2/4GnT, was identified by BLAST analysis of expressed sequence tags. The sequence of C2/4GnT encoded a putative type II transmembrane protein with significant sequence similarity to human C2GnT and IGnT. Expression of the secreted form of C2/4GnT in insect cells showed that the gene product had UDP-N-acetyl-α-d-glucosamine:acceptor β1,6-N-acetylglucosaminyltransferase (β1,6GlcNAc-transferase) activity. Analysis… 

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