Control of Lactate Production by Membrane Adenosine Triphosphatase Activity in Human Erythrocytes

  title={Control of Lactate Production by Membrane Adenosine Triphosphatase Activity in Human Erythrocytes},
  author={R. Whittam and M E Ager and James S. Wiley},
IT is generally believed from Schatzmann's1 work that lactate production in human erythrocytes is unaffected by ouabain and other cardiac glycosides which inhibit the active transport of sodium and potassium. The absence of a direct effect on energy production was further suggested by later studies on ion movements2,3. It has thus appeared that inhibition of active transport is unaccompanied by a change in energy production in spite of the smaller energy requirements of the cell. Active… 

Inhibition by Ouabain of Glycolysis in Ciliary Body

It has been shown that ouabain, a specific inhibitor of sodium–potassium ATPase, causes a 40 per cent decrease in the rate of oxygen uptake in kidney and in liver and a 30% decrease in brain.

Red Cell Glycolysis in the Myo dystrophic Child

The regulation of phosphoglycerate Kinase and pyruvate kinase in vivo followed a normal pattern, and sharp increases of fructose‐1,6‐diphosphate and dihydroxyacetone‐phosphate were observed in the normal cell.


It is concluded that the epithelial Na-K ATPase and the active cation transport system of the lens are identical or very closely related.

Ouabain and the distribution of calcium and magnesium in cerebral tissues in vitro

It is clear that in addition to its effects on transport of monovalent cations and on activity of Mg-dependent, Na-K-activated ATPase, ouabain can also affect the distribution of calcium and thereby respiratory metabolism in cerebral cortex.



Adenosinetriphosphatase activity and the active movements of alkali metal ions

The experiments to be described in the present paper were designed to see whether the red cell membrane possesses adenosinetriphosphatase activity and whether this ATP-ase activity is connected with the mechanisms responsible for ion transport.

The regulation of kidney respiration by sodium and potassium ions.

Adenosine-triphosphatase activity was measured in the same homogenate after diluting tenfold and allowing the coarse fibres to settle out, typical of four such experiments.

Respiratory control by an adenosine triphosphatase involved in active transport in brain cortex.

The immediate source of energy for the nerve impulse is the movement of Na+ and K+ ions down concentration gradients, one of the main roles of metabolism in nervous tissue to for the transfer of

Renal gluconeogenesis. The effect of diet on the gluconeogenic capacity of rat-kidney-cortex slices.

Measurements of the rates of gluconeogenesis from various precursors and the effects of the diet of rats and the composition of the medium on the rates are concerned.

The asymmetrical stimulation of a membrane adenosine triphosphatase in relation to active cation transport.

  • R. Whittam
  • Biology, Computer Science
    The Biochemical journal
  • 1962
The seaweed polysaccharide sulphate from Chondrus ocellatus (Mori, 1953) by Professor T. C. Mori and other seaweedpolysaccharides, heparins, potassium chondroitin sulphates and chemically sulphated hyaluronic acid by Dr W. J. Dyke.

The determination of lacic acid in microgram quantities.

Bacon, J. S. & Loxley, R. D. (1951).

Directional Effects of Alkali Metal Ions on Adenosine Triphosphate Hydrolysis in Erythrocyte Ghosts

Directional Effects of Alkali Metal Ions on Adenosine Triphosphate Hydrolysis in Erythrocyte Ghosts shows directional effects with respect to the concentration of adenosine and phosphate in the H2O2 mixture.

Potassium movements and ATP in human red cells

  • R. Whittam
  • Biology, Chemistry
    The Journal of physiology
  • 1958
The effects of cardiac glycosides show that ATP is not the only link, and are interpreted as indirect evidence for the participation of a membrane carrier in the linked transport of potassium with sodium across the cell membrane.