Control of Haemoglobin Synthesis: a Difference in the Size of the Polysomes making α and β Chains

@article{Hunt1968ControlOH,
  title={Control of Haemoglobin Synthesis: a Difference in the Size of the Polysomes making $\alpha$ and $\beta$ Chains},
  author={R. T. Hunt and Anthony Hunter and Alan J. Munro},
  journal={Nature},
  year={1968},
  volume={220},
  pages={481-483}
}
HAEMOGLOBIN is composed of equimolar amounts of α and β chains. Although it is not essential to synthesize the same number of molecules of each chain, this was originally thought to be the case1. The finding of a pool of α chains in rabbit reticulocytes implies that either at some stage there has been excess synthesis of α chains, or that the α chains are continually produced in excess of β chains2–4. An understanding of the mechanisms which control the balance of the synthesis of the two… 
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The major steps in haemoglobin synthesis in red cell precursors are now worked out and only details of specific mechanisms remain to be filled in. Thus the major steps in the production of a globin
THE CONTROL OF GLOBIN SYNTHESIS IN RABBIT RETICULOCYTES *
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  • Biology
    Annals of the New York Academy of Sciences
  • 1974
TLDR
The attention today is almost wholly focused on the problem of the mechanism and control of the initiation of protein synthesis since all the evidence points to this step as being subject to control.
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TLDR
The results suggest that reticulocytes, unlike nucleated cell lines, can coordinately reduce rates of initiation and elongation in response to certain physiological stresses.
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TLDR
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TLDR
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Translational control of hemoglobin synthesis in thalassemic bone marrow.
TLDR
In normal and beta-thalassemic marrow, as well as in reticulocytes, beta-chain production, though deficient, occurs predominantly on larger polysomes than the production of alpha-chains, and each beta- and delta-mRNA initiates protein synthesis at a rate faster than does each alpha-m RNA.
GLOBIN TRANSLATION IN THALASSEMIC BONE MARROW *
TLDR
Extending studies previously performed only in reticulocytes to bone marrow cells of B-thalassemia patients, in order to test the function of mRNA that is found there, shows a normal rate of initiation of P-mRNA translation.
Synthesis of rabbit globin by reticulocyte postribosomal supernatant and heterologous ribosomes.
TLDR
Cell-free protein synthesis has been studied in heterologous systems using rabbit reticulocyte postribosomal supernatant and ribosomes obtained from HeLa or mouse myeloma cells using rabbit hemoglobin and the preferential translation of mRNA present in thesupernatant, presumably in a ribonucleoprotein particle is discussed.
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References

SHOWING 1-8 OF 8 REFERENCES
Alpha-chain and globin: intermediates in the synthesis of rabbit hemoglobin.
TLDR
The synthesis of hemoglobin by rabbit reticulocytes has been studied by means of pulse-labelling and chase experiments and the existence of heme-containing intermediates has been obtained, suggesting that the same biosynthetic process is responsible for these rates.
Polyribosomal synthesis and assembly of the H and L chains of gamma globulin.
TLDR
Immunologic analysis indicated that these two polypeptides represented 20-30% of the total protein synthesized by both tumors, and techniques for separating and identifying the two chains affords a unique opportunity to study the synthesis and assembly of thepolypeptide chains of a disulfide-linked protein.
Rabbit Hemoglobin Biosynthesis: Use of Human Hemoglobin Chains To Study Molecule Completion
TLDR
A cell-free protein-synthesizing system made from rabbit reticulocytes was used to incorporate 14C-amino acids into hemoglobin, and a model of hemoglobin construction in which soluble alpha chains are intermediates is suggested.
A multiple ribosomal structure in protein synthesis.
  • J. Warner, P. Knopf, A. Rich
  • Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1963
Assembly of the peptide chains of hemoglobin.
  • H. Dintzis
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1961