Control of ATP concentration in Escherichia coli using synthetic small regulatory RNAs for enhanced S-adenosylmethionine production.

@article{Chen2015ControlOA,
  title={Control of ATP concentration in Escherichia coli using synthetic small regulatory RNAs for enhanced S-adenosylmethionine production.},
  author={Yawei Chen and Shuangyan Lou and Lihai Fan and Xin Zhang and Tianwei Tan},
  journal={FEMS microbiology letters},
  year={2015},
  volume={362 15},
  pages={fnv115}
}
ATP is the limiting precursor and driving force for S-adenosylmethionine (SAM) biosynthesis in Escherichia coli. In contrast to traditional optimization of fermentation processes, the synthetic sRNA-based repression strategy, which was developed as a highly efficient gene knockdown approach, has been applied for the regulation of the intracellular ATP concentration in order to enhance SAM production. In this work, proB, glnA and argB, all involved in the synthesis of ATP-dependent by-products… CONTINUE READING