Control and recognition of anionic ligands in myoglobin.

  title={Control and recognition of anionic ligands in myoglobin.},
  author={Francesca Cutruzzol{\`a} and C T Allocatelli and Paolo Ascenzi and Martino Bolognesi and Stephen G Sligar and Maurizio Brunori},
  journal={FEBS letters},
  volume={282 2},
Equilibrium and kinetic experiments on site-directed mutants of a synthetic sperm whale myoglobin (Mb) gene have been performed. Results on the reactivity on both ferric and ferrous wild type and mutants Mb's are presented. Analysis of ligand binding to His (E7) Val and His (E7) Val-Thr (E10) Arg mutants compared to wild-type sperm whale, horse and Aplysia limacina Mb's, shows that the introduction of an arginyl residue at the topological position E10 greatly enhances the stability of the… CONTINUE READING