Control analysis applied to single enzymes: can an isolated enzyme have a unique rate-limiting step?

@article{Brown1993ControlAA,
  title={Control analysis applied to single enzymes: can an isolated enzyme have a unique rate-limiting step?},
  author={Guy C Brown and Chris E. Cooper},
  journal={The Biochemical journal},
  year={1993},
  volume={294 ( Pt 1)},
  pages={87-94}
}
Control analysis is used to analyse and quantify the concept of a rate-limiting step within an enzyme. The extent to which each rate constant within the enzyme limits the steady-state rate of the enzyme and the levels of enzyme intermediate species are quantified as flux and concentration control coefficients. These coefficients are additive and obey summation theorems. The control coefficients of triose phosphate isomerase, carbamate kinase and lactate dehydrogenase are calculated from… CONTINUE READING