Contributions to somatic and germline lineages of chicken blastodermal cells maintained in culture.

@article{Etches1996ContributionsTS,
  title={Contributions to somatic and germline lineages of chicken blastodermal cells maintained in culture.},
  author={Robert J. Etches and Melody Clark and Amy Toner and Guisheng Liu and Ann M. Verrinder Gibbins},
  journal={Molecular reproduction and development},
  year={1996},
  volume={45 3},
  pages={291-8}
}
Chicken blastodermal cells were cultured for 48 hr as explanted intact embryos, as dispersed cells in a monolayer, or with a confluent layer of mouse fibroblasts. The cells were then dispersed and injected into stage X (E-G&K) recipient embryos that were exposed to 600 rads of irradiation from a 60Co source. Regardless of the conditions in which the cells were cultured, chimeras with contributions to both somatic tissues and the germline were observed. When blastodermal cells were co-cultured… CONTINUE READING

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