Contributions of supercoiling to Tn3 resolvase and phage Mu Gin site-specific recombination.

@article{Benjamin1996ContributionsOS,
  title={Contributions of supercoiling to Tn3 resolvase and phage Mu Gin site-specific recombination.},
  author={Keila Benjamin and A P Abola and Roland Kanaar and Nicholas R. Cozzarelli},
  journal={Journal of molecular biology},
  year={1996},
  volume={256 1},
  pages={50-65}
}
Members of the resolvase/invertase family of site-specific recombinases require supercoiled substrates containing two recombination sites. To dissect the roles of supercoiling in recombination by the Tn3 and gamma delta resolvases and the phage Mu Gin invertase, we used substrates that provided some but not all of the topological features of the standard substrate. We divided the Tn3 resolvase reaction into two stages, synapsis and postsynapsis. Using structural and functional topological… CONTINUE READING