Contributions Sarcoplasmic Reticulum Ca 21 Uptake and Thapsigargin Sensitivity in Permeabilized Rabbit and Rat Ventricular Myocytes

@inproceedings{HoveMadsen2005ContributionsSR,
  title={Contributions Sarcoplasmic Reticulum Ca 21 Uptake and Thapsigargin Sensitivity in Permeabilized Rabbit and Rat Ventricular Myocytes},
  author={L. Hove-Madsen and Donald M. Bers},
  year={2005}
}
Ca'+ uptake by the sarcoplasmic reticulum (SR) and free [Ca2"] were measured simultaneously with indo 1 and a Ca'+-selective minielectrode in suspensions of permeabilized rabbit or rat ventricular myocytes ('-'10 mg/mL protein). In the presence of 25 ,umol/L ruthenium red and 10 mmol/L oxalate, the K. for Ca2 uptake by the SR was '250 nmol/L in rabbit and rat ventricular myocytes. The maximal Ca ` uptake rate was 2.4 times higher in rat than in rabbit. Addition of 5 nmol thapsigargin (TG) per… CONTINUE READING