Contribution of water molecules in the interior of a protein to the conformational stability.

@article{Takano1997ContributionOW,
  title={Contribution of water molecules in the interior of a protein to the conformational stability.},
  author={Kazufumi Takano and Jun Funahashi and Yuriko Yamagata and Shingo Fujii and Katsuhide Yutani},
  journal={Journal of molecular biology},
  year={1997},
  volume={274 1},
  pages={132-42}
}
Water molecules frequently occur in the interior of globular proteins. To elucidate the contribution of buried water molecules to the conformational stability of a protein, we examined the crystal structures and the thermodynamic parameters of denaturation of six Ile to Ala/Gly mutant human lysozymes, in which a cavity is created at each mutation site by the substitution of a smaller side-chain for a larger one. One or two ordered water molecules were found in the cavities created in some… CONTINUE READING