The meniscofemoral ligament (MFL) is a major structure in the posterior aspect of the porcine knee together with the posterior cruciate ligament (PCL). While the porcine knee is a frequently used animal model for biomechanical evaluation of PCL reconstruction techniques, the contribution of the MFL to stability of the porcine knee is not well understood. The purpose of this study is (1) to evaluate the kinematics of the knee after sequential cutting of the PCL and MFL and (2) to determine the in situ forces of the PCL and MFL in response to a posterior tibial load of 89 N using the robotic/universal force-moment sensor system from 15° to 90° of knee flexion. Ten porcine knees were used in this study. The magnitude of posterior tibial translation under a posterior tibial load was significantly increased (P < 0.01) after sequential transection of the PCL and the MFL at each testing angle compared to the intact condition. The in situ force of the PCL was highest at 60° of flexion (82.3 ± 8.6 N) and lowest at 15° of flexion (45.1 ± 15.9 N). The in situ force of the MFL was highest at 15° of flexion (24.3 ± 6.5 N) and lowest at 90° of flexion (12.9 ± 10.5 N). The findings in this study revealed a biomechanical contribution of the MFL as the secondary restraint to the posterior tibial translation in conjunction with the PCL especially near full extension.