Contribution of the beta subunit M2 segment to the ion-conducting pathway of the acetylcholine receptor.

@article{Zhang1998ContributionOT,
  title={Contribution of the beta subunit M2 segment to the ion-conducting pathway of the acetylcholine receptor.},
  author={Hailan Zhang and A. Karlin},
  journal={Biochemistry},
  year={1998},
  volume={37 22},
  pages={7952-64}
}
We have applied the substituted-cysteine-accessibility method (SCAM) to the M2 segment and the M1-M2 loop of the acetylcholine (ACh) receptor beta subunit. Each residue from beta P248 to beta D273 was mutated one at a time to Cys, and the mutant beta subunits were expressed together with wild-type alpha, beta, and delta subunits in Xenopus oocytes. For each of the mutants, the ACh-induced current was near wild-type. The accessibility of the substituted Cys was inferred from the irreversible… CONTINUE READING

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