Contribution of flavin-containing monooxygenase and cytochrome P450 to imipramine N-oxidation in rat hepatic microsomes.

@article{Narimatsu1999ContributionOF,
  title={Contribution of flavin-containing monooxygenase and cytochrome P450 to imipramine N-oxidation in rat hepatic microsomes.},
  author={Shizuo Narimatsu and Shigeo Yamamoto and Ryuichi Kato and Yasuhiro Masubuchi and Toshiharu Horie},
  journal={Biological & pharmaceutical bulletin},
  year={1999},
  volume={22 6},
  pages={567-71}
}
Enzymatic formation of desipramine (DMI) and imipramine N-oxide (IMINO) was kinetically characterized in rat liver microsomes at pH 8.5 and 7.5. The formation of IMINO was quickly suppressed by the preincubation of microsomes at 37 degrees C at pH 8.5, but the suppression was comparatively gentle at pH 7.5. In kinetic studies, the formation of DMI was monophasic at the two pH points, and a substrate inhibition was observed at pH 8.5, but not at pH 7.5. In contrast, the formation of IMINO was… CONTINUE READING