Contribution of exogenous substrates to acetyl coenzyme A: measurement by 13C NMR under non-steady-state conditions.

@article{Malloy1990ContributionOE,
  title={Contribution of exogenous substrates to acetyl coenzyme A: measurement by 13C NMR under non-steady-state conditions.},
  author={Craig Malloy and James R. Thompson and F. M. H. Jeffrey and A Dean Sherry},
  journal={Biochemistry},
  year={1990},
  volume={29 29},
  pages={6756-61}
}
A method is presented for the rapid determination of substrate selection in a manner that is not restricted to conditions of metabolic and isotopic steady state. Competition between several substrates can be assessed directly and continuously in a single experiment, allowing the effect of interventions to be studied. It is shown that a single proton-decoupled 13C NMR spectrum of glutamate provides a direct measure of the contribution of exogenous 13C-labeled substrates to acetyl-CoA without… CONTINUE READING
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