Contribution of calpain Lp82-induced proteolysis to experimental cataractogenesis in mice.

  title={Contribution of calpain Lp82-induced proteolysis to experimental cataractogenesis in mice.},
  author={Yoshikuni Nakamura and Chiho Fukiage and Marjorie Shih and Hong Ma and Larry L. David and Mitsuyoshi Azuma and Thomas R. Shearer},
  journal={Investigative ophthalmology & visual science},
  volume={41 6},
PURPOSE The purpose of the present experiments was to provide a biochemical mechanism for the involvement of lens-specific calpain Lp82 in experimental cataractogenesis in mice. METHODS Nuclear cataracts were produced by culturing lenses from 4-week-old mice and rats in calcium ionophore A23187 or by injection of buthionine sulfoximine (BSO) into 7-day-old mice. Casein zymography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblot analysis, calcium determinations, in vitro… CONTINUE READING


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