Contribution of anaphase B to chromosome separation in higher plant cells estimated by image processing.

@article{Hayashi2007ContributionOA,
  title={Contribution of anaphase B to chromosome separation in higher plant cells estimated by image processing.},
  author={Tomomi Hayashi and Toshio Sano and Natsumaro Kutsuna and Fumi Kumagai-Sano and Seiichiro Hasezawa},
  journal={Plant & cell physiology},
  year={2007},
  volume={48 10},
  pages={1509-13}
}
Anaphase can be categorized into the two subphases of anaphase A and B, but anaphase B has not been clearly described in higher plant cells. In this study, we time-sequentially followed the dynamics of chromosome segregation and spindle elongation in tobacco BY-2 cells using histone-red fluorescent protein (RFP) and green fluorescent protein (GFP)-tubulin, respectively. Construction of kymographs and determination of the positions of chromosomes and spindle edges by image processing revealed… CONTINUE READING
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