Contribution of a salt bridge to the thermostability of DNA binding protein HU from Bacillus stearothermophilus determined by site-directed mutagenesis.


We have employed site-directed mutagenesis to evaluate the contribution of the amino acid residues, Glu34, Arg37, and Lys38, to the thermostability of the thermophilic protein, Bacillus stearothermophilus DNA binding protein HU (BstHU), relative to the mesophilic homologue, Bacillus subtilis HU (BsuHU). Mutants BstHU-E34D and BstHU-K38N, in which Glu34 and… (More)


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