Contribution of TNF-α Converting Enzyme and Proteinase-3 to TNF-α Processing in Human Alveolar Macrophages

@article{Armstrong2006ContributionOT,
  title={Contribution of TNF-$\alpha$ Converting Enzyme and Proteinase-3 to TNF-$\alpha$ Processing in Human Alveolar Macrophages},
  author={Lynne Armstrong and Sofia I. H. Godinho and Kay M. Uppington and H A Whittington and Ann B. Millar},
  journal={American Journal of Respiratory Cell and Molecular Biology},
  year={2006},
  volume={34},
  pages={219-225}
}
Membrane-associated TNF-alpha cleavage is required to yield the 17.5-kD soluble product. This process is poorly understood in human cells, and no studies have related this process to the alveolar macrophage (AM). TNF-alpha-converting enzyme (TACE) is known to cleave TNF at the Ala-76-Val-77 site. We have evaluated the expression, regulation, and catalytic function of TACE in healthy human AMs. TACE was detected on the surface of AMs using flow cytometry. TACE protein can be upregulated by LPS… 
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