Contribution of Interaction between Nitric Oxide and Cyclooxygenases to the Production of Prostaglandins in Carrageenan-induced Inflammation

  title={Contribution of Interaction between Nitric Oxide and Cyclooxygenases to the Production of Prostaglandins in Carrageenan-induced Inflammation},
  author={Masaki Toriyabe and Keiichi Omote and Tomoyuki Kawamata and Akiyoshi Namiki},
Background:Nitric oxide (NO) and prostaglandins (PGs) are crucial mediators contributing to generation of inflammatory responses and pain. This study was designed to investigate the effects of peripherally released NO on cyclooxygenase (COX) expression/activation and production of PGs in carrageenan-induced inflammation. Methods:A microdialysis probe was implanted subcutaneously into the skin of hind paws of rats. The concentrations of NO metabolites, PGE2, and 6-keto-PGF1&agr; (metabolite of… 

COX-2 expression and function in the hyperalgesic response to paw inflammation in mice.

Sensory and signaling mechanisms of bradykinin, eicosanoids, platelet-activating factor, and nitric oxide in peripheral nociceptors.

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We have recently put forward the hypothesis that the dual inhibition of proinflammatory nitric oxide (NO) and prostaglandins (PG) may contribute to the antiinflammatory properties of nitric oxide

Peripheral nitric oxide in carrageenan-induced inflammation

Pharmacological analysis of cyclooxygenase-1 in inflammation.

  • C. SmithY. Zhang P. Isakson
  • Medicine, Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1998
The results suggest that, in addition to the role of peripherally produced PGs, there is a critical, centrally mediated neurological component to inflammatory pain that is mediated at least in part by COX-2.

Nitric oxide activates cyclooxygenase enzymes.

It is demonstrated that NO enhances COX activity through a mechanism independent of cGMP and suggested that, in conditions in which both the NOS and COX systems are present, there is an NO-mediated increase in the production of proinflammatory prostaglandins that may result in an exacerbated inflammatory response.

Effects of nitric oxide and nitric oxide‐derived species on prostaglandin endoperoxide synthase and prostaglandin biosynthesis

This study has shown that removal of O2·− from RAW 267.4 cells that produce NO· and PGHS inhibits prostaglandin biosynthesis to the same extent as NOS inhibitors.

Nitric oxide: a key mediator in the early and late phase of carrageenan‐induced rat paw inflammation

The results suggest that the NO produced by cNOS is involved in the development of inflammation at early time points following carrageenan administration and that No2−/NO3− or PGE2 production in the paw exudate is involvedin the maintenance of the inflammatory response at later time points.

Co-regulation between cyclo-oxygenase-2 and inducible nitric oxide synthase expression in the time-course of murine inflammation

The results indicate that PGE2 and NO may play in vivo mutual modulatory roles in the inflammatory response caused by zymosan injection into the mouse air pouch, a suitable model to study drugs acting on those pathways.

Role of Prostaglandin Receptor EP1 in the Spinal Dorsal Horn in Carrageenan-induced Inflammatory Pain

It seems that some of the mechanisms underlying inflammation-induced plastic changes are mediated by time-dependent increase in PGE2 concentration, activation of EP1 receptors, and increase in [Ca2+]i in the spinal dorsal horn.

Selective inhibition of cyclooxygenase (COX)-2 reverses inflammation and expression of COX-2 and interleukin 6 in rat adjuvant arthritis.

It is suggested that COX-2 plays a prominent role in the inflammation associated with adjuvant arthritis and that COx-2 derived PGs upregulate CO X-2 and IL-6 expression at inflammatory sites.

Regulation of the expression of cyclooxygenase-2 by nitric oxide in rat peritoneal macrophages.

Results provide evidence that NO participates in PG production by negative regulation of Cox-2 expression, and addition of NO donors reversed the effects of L-NMMA, confirming the role of NO on Cox-1 expression.