Contraction of gut smooth muscle cells assessed by fluorescence imaging.

@article{Tokita2015ContractionOG,
  title={Contraction of gut smooth muscle cells assessed by fluorescence imaging.},
  author={Yohei Tokita and Hirotada Akiho and Kazuhiko Nakamura and Eikichi Ihara and Masahiro Yamamoto},
  journal={Journal of pharmacological sciences},
  year={2015},
  volume={127 3},
  pages={344-51}
}
Here we discuss the development of a novel cell imaging system for the evaluation of smooth muscle cell (SMC) contraction. SMCs were isolated from the circular and longitudinal muscular layers of mouse small intestine by enzymatic digestion. SMCs were stimulated by test agents, thereafter fixed in acrolein. Actin in fixed SMCs was stained with phalloidin… CONTINUE READING