Continuous uninterrupted single medium culture without medium renewal versus sequential media culture: a sibling embryo study.

Abstract

Eight hundred ninety-three sibling embryos from 80 IVF cycles were cultured side by side in either: 1) a single medium continuously, without medium renewal on day 3; or 2) sequential media. There were no significant differences between the two culture media systems regarding embryo quality or the proportion of embryos selected for transfer on day 3 from either media; however, for day 5 embryo transfer, a greater number of blastocysts were available, and were selected for transfer, from the continuous single medium culture compared with sequential media culture.

DOI: 10.1016/j.fertnstert.2009.05.008
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@article{Reed2009ContinuousUS, title={Continuous uninterrupted single medium culture without medium renewal versus sequential media culture: a sibling embryo study.}, author={Michael L. Reed and Amanda Hamic and Douglas J Thompson and Charles L Caperton}, journal={Fertility and sterility}, year={2009}, volume={92 5}, pages={1783-6} }