Continuous registration of thrombin generation in plasma, its use for the determination of the thrombin potential.

  title={Continuous registration of thrombin generation in plasma, its use for the determination of the thrombin potential.},
  author={H Coenraad Hemker and Simone J H Wielders and Hon Kessels and Suzette B{\'e}guin},
  journal={Thrombosis and haemostasis},
  volume={70 4},
A method is described by which the time-course of thrombin generation in plasma can be obtained from a continuous optical density recording of p-nitroaniline (pNA) production in a 2:3 diluted plasma. A chromogenic substrate, methylmalonyl-methylanalyl-arginyl-pNA (SQ 68), is used that is specifically split by thrombin but at a low rate. The thrombin that appears and disappears in the plasma does not split more than 5% of the substrate added, so the rate of substrate conversion is in good… CONTINUE READING

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