Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer

  title={Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer},
  author={Ulrich Schreiber and Ulrich Schliwa and Wolfgang Bilger},
  journal={Photosynthesis Research},
A newly developed fluorescence measuring system is employed for the recording of chlorophyll fluorescence induction kinetics (Kautsky-effect) and for the continuous determination of the photochemical and non-photochemical components of fluorescence quenching. The measuring system, which is based on a pulse modulation principle, selectively monitors the fluorescence yield of a weak measuring beam and is not affected even by extremely high intensities of actinic light. By repetitive application… 

Rapidly reversible chlorophyll fluorescence quenching induced by pulses of supersaturating light in vivo

A special type of non-photochemical quenching, HIQ, is reported, which is rapidly induced by a pulse of high-intensity light, when PS II reaction centers are closed, and rapidly relaxes again after the pulse, in line with the working hypothesis of HIQ being caused by the annihilation of singlet excited chlorophyll a by triplet excited carotenoid.

New Ways of Assessing Photosynthetic Activity with a Pulse Modulation Fluorometer

The special features of a new type of modulation fluorometer (PAM Fluorometer) are outlined in comparison to conventional measuring systems. Fluorescence is excited by 1 µs pulses of a

Kinetic imaging of chlorophyll fluorescence using modulated light

The fluorometer produces dynamic images of chlorophyll fluorescence from leaves or plants, providing accurate mapping of F0 and FV, and non-photochemical quenching and can record fluorescence images of leaves in daylight under field conditions.

The relationship between non-photochemical quenching of chlorophyll fluorescence and the rate of photosystem 2 photochemistry in leaves

It is demonstrated that both the high-energy state and the more slowly relaxing components of non-photochemical quenching reduce the rate of PS 2 photochemistry.

Light-induced heat production correlated with fluorescence and its quenching mechanisms

The results demonstrate that the changes of the photoacoustic signal can in most cases be related directly or indirectly to changes in the photochemical activity and that the sum of energy dissipation processes resulting in the production of light-induced heat and measured by the high-frequency photoac acoustic signal must be taken into account when judging photosynthetic activity.

An Analysis of the Mechanism of the Low-wave Phenomenon of Chlorophyll Fluorescence

It is suggested that low waves of chlorophyll fluorescence are induced by increased non-photochemical quenching, as a result of the formation of a trans-thylakoid proton gradient due to cyclic electron flow around PS I.

A Microscope for Two-Dimensional Measurements of In Vivo Chlorophyll Fluorescence Kinetics Using Pulsed Measuring Radiation, Continuous Actinic Radiation, and Saturating Flashes

Transients of chlorophyll fluorescence in photosynthetic objects are often measured using short pulses of exciting radiation, which has recently been employed to capture kinetic images of

On the relationship between the non-photochemical quenching of the chlorophyll fluorescence and the Photosystem II light harvesting efficiency. A repetitive flash fluorescence induction study

Several mechanisms that modulate the fluorescence emission in parallel to the QA redox state and to the membrane energization were resolved and classified in relation to the light harvesting capacity of Photosystem II.

Measurement of chlorophyll fluorescence within leaves using a modified PAM Fluorometer with a fiber-optic microprobe

The new system employs a miniature photomultiplier for detection of the pulse-modulated fluorescence signal received by the 20 μm fiber tip and qualifies for recordings of fluorescence induction kinetics (Kautsky effect), fluorescence quenching by the saturation pulse method and determination of quantum yield of energy conversion at Photosystem II at different sites within a leaf.

‘Low-waves>’ in chlorophyll fluorescence kinetics indicate deprivation of bicarbonate*

The results suggest that low-waves in chlorophyll fluorescence kinetics indicate deprivation of bicarbonate in the reaction centre of Photosystem II.




Analysis is made of the changes in the redox state of PS2 electron acceptors and the distribution of excitation energy between PS1 and PS2 during the fluorescence induction curve from pea leaves to allow the quantitation of photochemical and non-photochemical components of fluorescence quenching throughout the curve to be made.

Chlorophyll fluorescence yield changes as a tool in plant physiology I. The measuring system

  • U. Schreiber
  • Environmental Science
    Photosynthesis Research
  • 2004
A brief survey of the different possibilities to measure Chl fluorescence changes is given and emphasis will be put on such techniques which can be applied in plant physiological work.

Studies on the induction of chlorophyll fluorescence in barley protoplasts. II. Resolution of fluorescence quenching by redox state and the transthylakoid pH gradient

  • W. QuickP. Horton
  • Environmental Science
    Proceedings of the Royal Society of London. Series B. Biological Sciences
  • 1984
During the chlorophyll fluorescence oscillation described in barley protoplasts (Quick & Horton, Proc. R. Soc. Lond. B 220, 361-370, 1984) the components which contribute to quenching have been

Relations between electron transport and carbon assimilation; simultaneous measurement of chlorophyll fluorescence, transthylakoid pH gradient and O2 evolution in isolated chloroplasts

  • P. Horton
  • Environmental Science
    Proceedings of the Royal Society of London. Series B. Biological Sciences
  • 1983
An apparatus is described that allows simultaneous measurement of photosynthetic O2 evolution, chlorophyll fluorescence and the transthylakoid pH gradient (∆pH) in isolated chloroplasts irradiated

The relationship between the redox state of QA and photosynthesis in leaves at various carbon-dioxide, oxygen and light regimes

Both at low and high fluence rates, the redox state of QA was little altered when the CO2 concentration was reduced from saturation to about 600 μl·l-1 although photosynthesis was decreased particularly at high Fluence rates.