Continuous purification of antibodies from cell culture supernatant with aqueous two-phase systems: from concept to process.

@article{Rosa2013ContinuousPO,
  title={Continuous purification of antibodies from cell culture supernatant with aqueous two-phase systems: from concept to process.},
  author={Paula A J Rosa and Ana Margarida Azevedo and Sandra Sommerfeld and Martina Mutter and Werner B{\"a}cker and Maria Raquel Aires-Barros},
  journal={Biotechnology journal},
  year={2013},
  volume={8 3},
  pages={352-62}
}
An aqueous two-phase extraction (ATPE) process based on a PEG/phosphate system was developed for the capture of human immunoglobulin G and successfully applied to a Chinese hamster ovary and a PER.C6® cell supernatant. A continuous ATPE process incorporating three different steps (extraction, back-extraction, and washing) was set up and validated in a pump mixer-settler battery. Most of the higher molecular weight cell supernatant impurities were removed during the extraction step, while most… CONTINUE READING