Continuous production of scopolamine by a culture of Duboisia leichhardtii hairy root clone in a bioreactor system

@article{Muranaka2004ContinuousPO,
  title={Continuous production of scopolamine by a culture of Duboisia leichhardtii hairy root clone in a bioreactor system},
  author={Toshiya Muranaka and Hideo Ohkawa and Yasuyuki Yamada},
  journal={Applied Microbiology and Biotechnology},
  year={2004},
  volume={40},
  pages={219-223}
}
The scopolamine-releasing hairy root clone DL47-1 of Duboisia leichhardtii was cultured in an Amberlite XAD-2 column-combined bioreactor system for continuous production of scopolamine. The medium used was continuously exchanged during culture to maintain the electrical conductivity of the medium constant. After culturing the hairy roots in the system for 11 weeks, 0.5 g/l of scopolamine was obtained in the column. When the roots were cultures in the reactor system containing polyurethane foam… 
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The hairy root clones of Duboisia leichhardtii were found to release scopolamine into the medium andScopolamine was recovered as the hydrobromide salt with more than 90% purity.
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