Continuous porcine cell lines developed from alveolar macrophages: partial characterization and virus susceptibility.

@article{Weingartl2002ContinuousPC,
  title={Continuous porcine cell lines developed from alveolar macrophages: partial characterization and virus susceptibility.},
  author={Hana M. Weingartl and M. Wildan Nasution Sabara and J Pasick and Elaine A Van Moorlehem and Lorne A. Babiuk},
  journal={Journal of virological methods},
  year={2002},
  volume={104 2},
  pages={
          203-16
        }
}
Porcine monomyeloid cell lines were established following transfection of primary porcine alveolar macrophage cultures with plasmid pSV3neo, carrying genes for neomycin resistance and SV40 large T antigen. The parental clone 3D4 exhibited a relatively rapid doubling time (25.5 h), high plating efficiency and mixed phenotype with respect to growth on a solid support. Single cell cloning of the 3D4 parent resulted in establishment of several cell lines; three of them designated 3D4/2, 3D4/21 and… CONTINUE READING
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