Continuous perfusion of mammalian cells embedded in agarose gel threads.

@article{Foxall1984ContinuousPO,
  title={Continuous perfusion of mammalian cells embedded in agarose gel threads.},
  author={D L Foxall and Jack S. Cohen and James B. Mitchell},
  journal={Experimental cell research},
  year={1984},
  volume={154 2},
  pages={521-9}
}
A method for perfusing cells by embedding them in fine agarose gel threads is described and characterized. The rate of diffusion of a metabolite into the gel threads is determined by 31P-NMR spectroscopy. This perfusion method is shown to enable Chinese hamster lung fibroblasts (CHLF) to remain in a metabolically active state with high levels of intracellular ATP for many hours. 

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