Continuous monitoring of intracerebral glutamate levels in awake monkeys using microdialysis and enzyme fluorometric detection.


A technique for continuous on-line detection of glutamate using brain microdialysis in awake primates is described. The method is based on an enzymatic assay using fluorescence detection of glutamate. The time resolution of the continuous fluorescent readout compares favorably with that of most published studies, which have used standard high-pressure… (More)