Continuous matrix-assisted refolding of proteins.

  title={Continuous matrix-assisted refolding of proteins.},
  author={Robert Schlegl and G{\"u}nter Iberer and Christine Machold and Roman Necina and Alois Jungbauer},
  journal={Journal of chromatography. A},
  volume={1009 1-2},
A refolding reactor was developed for continuous matrix-assisted refolding of proteins. The reactor was composed of an annular chromatography system and an ultrafiltration system to recycle aggregated proteins produced during the refolding reaction. The feed solution containing the denatured protein was continuously fed to the rotating bed perfused with buffer promoting folding of the protein. As the protein passed through the column, it was separated from chaotropic and reducing agents and the… CONTINUE READING
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