Continuous in vitro cultivation of Babesia caballi in serum-free medium

  title={Continuous in vitro cultivation of Babesia caballi in serum-free medium},
  author={Erich P. Zweygarth and Carmen van Niekerk and Danita de Waal},
  journal={Parasitology Research},
Experiments were undertaken to develop a serum-free medium for the in vitro cultivation of Babesia caballi, a tick-borne hemoprotozoan parasite, one of the causative agents of equine piroplasmosis. A modified HL-1 medium supplemented with horse serum, L-glutamine, antibiotics, and hypoxanthine was used. B. caballi organisms were continuously cultivated at 37 °C in microaerophilous stationary-phase culture in a humidified atmosphere containing 5% CO2 in air before exposure to serum-free culture… CONTINUE READING
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