Continuous-exchange cell-free protein synthesis using PCR-generated DNA and an RNase E-deficient extract.

@article{Jun2008ContinuousexchangeCP,
  title={Continuous-exchange cell-free protein synthesis using PCR-generated DNA and an RNase E-deficient extract.},
  author={Soo Youn Jun and Sang Hyeon Kang and Kwang-Ho Lee},
  journal={BioTechniques},
  year={2008},
  volume={44 3},
  pages={387-91}
}
Though the use of PCR-generated DNA (i.e., linear template) as template DNA is desirable because of its simple preparation, the linear template has not been routinely used in a conventional continuous-exchange cell-free (CECF) protein synthesis system due to the instability of the linear template and/or its transcript in the relatively long operation period. To overcome this problem and enhance soluble protein yield, an RNase E-deficient and molecular chaperone-enriched extract was used: (i… CONTINUE READING

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Molecular Cloning, 2nd ed

J. Sambrook, E. F. Fritsch, T. Maniatis
1989

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