Continuous enzyme-coupled assay for microbial transglutaminase activity.

  title={Continuous enzyme-coupled assay for microbial transglutaminase activity.},
  author={Samuel K. Oteng-Pabi and Jeffrey W Keillor},
  journal={Analytical biochemistry},
  volume={441 2},
Transglutaminases (protein-glutamine:amine γ-glutamyltransferase, EC are a family of calcium-dependent enzymes that catalyze an acyl transfer between glutamine residues and a wide variety of primary amines. When a lysine residue acts as the acyl-acceptor substrate, a γ-glutamyl-ε-lysine isopeptide bond is formed. This isopeptide bond formation represents protein cross-linking, which is critical to several biological processes. Microbial transglutaminase (mTG) is a bacterial variant of… CONTINUE READING


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