Continuous dialysis of cultures of human diploid cells (MRC 5) grown on microcarriers


We have shown that if the medium supplemented with foetal calf serum (FCS) is continuously dialysed against a large volume of medium without FCS, the growth of human diploid fibroblasts on microcarriers is more rapid and yields are more consistent. This approach allows a considerable saving of serum. 
DOI: 10.1007/BF01134596


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