Continuous cultures of fused cells secreting antibody of predefined specificity

  title={Continuous cultures of fused cells secreting antibody of predefined specificity},
  author={G. K{\"o}hler and C. Milstein},
THE manufacture of predefined specific antibodies by means of permanent tissue culture cell lines is of general interest. There are at present a considerable number of permanent cultures of myeloma cells1,2 and screening procedures have been used to reveal antibody activity in some of them. This, however, is not a satisfactory source of monoclonal antibodies of predefined specificity. We describe here the derivation of a number of tissue culture cell lines which secrete anti-sheep red blood… Expand
Production of antibodies of monoclonal specificity without the use of hybridoma cell lines
Reproducible immunodiagnostic tests require antibodies of standardized specificity and affinity. A significant step toward this goal has been achieved with the development of the methodology ofExpand
Immunization and fusion protocols for hybridoma production.
  • J. Wood
  • Biology, Medicine
  • Methods in molecular biology
  • 1984
Kohler and Milsteins' technique of monoclonal antibody production is now being exploited in most areas of biology, to immortalize and then select for clones of plasma cells secreting antibody against a desired antigen. Expand
Production of hybrid lines secreting monoclonal anti-idiotypic antibodies by cell fusion on membrane filters.
This method describes this method, analyze some parameters which control its efficiency for the production of intraspecific and interspecific hybrids and demonstrates that it can be successfully utilized for the isolation of hybridomas secreting anti-idiotypic antibodies. Expand
Production of Monoclonal Antibodies
  • W. Yokoyama
  • Medicine, Chemistry
  • Current protocols in cell biology
  • 2001
This unit describes the production of monoclonal antibodies beginning with basic protocols for immunization and cell fusion and selection and support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity. Expand
Antibodies to major histocompatibility antigens produced by hybrid cell lines
FUSION between myeloma cells and spleen cells from immunised donors has been shown to be a successful method of deriving homogeneous anti-SRBC (anti-sheep red blood cell) and anti-TNP antibodies1,2.Expand
Production of Monoclonal Antibodies
Monoclonal antibodies are the secreted products of a hybridoma cell line that is created from the fusion of an antibody-producing lymphocyte and a histocompatible myeloma cell. The hybrid cellExpand
A better cell line for making hybridomas secreting specific antibodies
The identification of such a cell line, Sp2/0-Ag14, is reported here the identification of a tumour cell fusion partner that makes no Ig but which can nevertheless be fused with spleen cells to obtain hybrids secreting only the specific antibody. Expand
Production of monoclonal antibodies reacting with the cytoplasm and surface of differentiated cells.
This chapter describes the techniques that have been successful in creating monoclonal antibodies to neuronal T cell, islet cell and thymic cell antigens. Expand
The growth of publications on monoclonal antibodies 1975–1981
The aim was to show the rise in publications since 1975 on the one hand and to find the most important journals containing articles on this subject on the other hand, based on an online search in the US database BIOSIS. Expand
Monoclonal antibodies derived from hybrid myelomas
  • M. Clark
  • Biology, Medicine
  • La Ricerca in clinica e in laboratorio
  • 1981
Production of human monoclonal antibodies is at present limited by the lack of suitable parental myeloma cell lines although some success has been reported using both mouse and human cell lines. Expand


Mouse myelomas and lymphomas in culture.
Assays of the growth inhibiting activities of thymidine and hydrocortisone show that the lymphoma cells are much more sensitive than the myeloma cells, suggesting that in these respects the cultured cell lines possess some of the differentiated characteristics of normal lymphocytes and plasma cells. Expand
Somatic mutation and the origin of antibody diversity. Clonal variability of the immunoglobulin produced by MOPC 21 cells in culture
A method has been developed for the screening of immunoglobulins produced by large numbers of individual clones of the mouse myeloma in tissue culture using autoradiography to detect variations between different clones. Expand
Fusion of Two Immunoglobulin-producing Myeloma Cells
EACH immunoglobulin chain is the integrated expression of one of several V and C genes, coding respectively for their variable and constant sections1. The restricted expression of these genes leadsExpand
Pattern of immunoglobulin synthesis and assembly in a human-mouse somatic cell hybrid clone.
  • J. Schwaber, E. Cohen
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1974
Analysis of fusion of human peripheral blood lymphocytes with mouse myeloma cells yielded a somatic cell hybrid clone that secreted both human and mouse immunoglobulins that synthesized human gamma and alpha heavy chains and human light chains, as well as mouse alphaheavy chains and mouse light chains. Expand
Plaque Formation in Agar by Single Antibody-Producing Cells
Distinct plaques, each of which is due to the release of hemolysin by a single antibody-forming cell, are revealed by complement after incubation, in an agar layer, of a mixture of sheep red cellsExpand
Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed Recombinants
Evidence can be obtained which suggests that mating may be followed by segregation in mice, and when two clonal lines of mouse fibroblasts are grown together for 4 days, hybrid cells can be detected by selective conditions. Expand
The complete amino acid sequence of a mouse ϰ light chain
The complete amino acid sequence of the kappa-chain of the mouse myeloma protein MOPC 21 was established. The protein was reduced and alkylated with iodo[2-(14)C]acetic acid, and 21 tryptic peptidesExpand
Progress in immunology. Syndromes of diminished resistance to infection.
In this review of progress in immunology, an attempt is made to synthesize some of the rapidly accumulating observations in clinical medicine and experimental biology into a workable scheme, whichExpand
Partial Deletion in the Heavy Chain Disease Protein ZUC
The sequence of the first fifty-five residues of protein ZUC (γ3 subclass) is presented and a stretch of the myeloma protein KUP which includes the five interchain S—S bonds characteristic of IgG3 proteins7 is compared. Expand
Isoelectric Focusing in Polyacrylamide Gel and its Application to Immunoglobulins
In this form the technique has been successfully applied to studies on the heterogeneity of myoglobins, cytochrome c6, lactoperoxidase7 and a number of other proteins (see review by Haglund8). Expand