Continuous cultures of fused cells secreting antibody of predefined specificity

@article{Khler1975ContinuousCO,
  title={Continuous cultures of fused cells secreting antibody of predefined specificity},
  author={G. K{\"o}hler and C. Milstein},
  journal={Nature},
  year={1975},
  volume={256},
  pages={495-497}
}
THE manufacture of predefined specific antibodies by means of permanent tissue culture cell lines is of general interest. There are at present a considerable number of permanent cultures of myeloma cells1,2 and screening procedures have been used to reveal antibody activity in some of them. This, however, is not a satisfactory source of monoclonal antibodies of predefined specificity. We describe here the derivation of a number of tissue culture cell lines which secrete anti-sheep red blood… Expand
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The aim was to show the rise in publications since 1975 on the one hand and to find the most important journals containing articles on this subject on the other hand, based on an online search in the US database BIOSIS. Expand
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  • Biology, Medicine
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  • 1981
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