Continuous butanol production with reduced byproducts formation from glycerol by a hyper producing mutant of Clostridium pasteurianum

  title={Continuous butanol production with reduced byproducts formation from glycerol by a hyper producing mutant of Clostridium pasteurianum},
  author={Alok Malaviya and Yu-Sin Jang and Sang Yup Lee},
  journal={Applied Microbiology and Biotechnology},
Butanol, a four-carbon primary alcohol (C4H10O), is an important industrial chemical and has a good potential to be used as a superior biofuel. Bio-based production of butanol from renewable feedstock is a promising and sustainable alternative to substitute petroleum-based fuels. Here, we report the development of a process for butanol production from glycerol, which is abundantly available as a byproduct of biodiesel production. First, a hyper butanol producing strain of Clostridium… Expand
Comprehensive investigations of biobutanol production by a non-acetone and 1,3-propanediol generating Clostridium strain from glycerol and polysaccharides
The wild-type strain GL11 was found to be particularly advantageous due to its capability of efficient butanol production from glycerol and polysaccharides with elimination of acetone and 1,3-PDO formation and the highbutanol production with in situ extraction by using biodiesel would significantly enhance the economic feasibility of fermentative production of butanol from glycersol. Expand
Production of 1,3-PDO and butanol by a mutant strain of Clostridium pasteurianum with increased tolerance towards crude glycerol
Utilizing stored crude glycerol, the mutant strain showed significantly increased rates compared to the wild type and high yields of the main products (butanol and 1,3-PDO) were detected and these two products were efficiently separated in two steams using gas-stripping. Expand
Clostridium sp. as Bio-Catalyst for Fuels and Chemicals Production in a Biorefinery Context
Clostridium sp. is a genus of anaerobic bacteria capable of metabolizing several substrates (monoglycerides, diglycerides, glycerol, carbon monoxide, cellulose, and more), into valuable products.Expand
Modulation of crude glycerol fermentation by Clostridium pasteurianum DSM 525 towards the production of butanol
Abstract High production yields and productivities are requisites for the development of an industrial butanol production process based on biodiesel-derived crude glycerol. However, impuritiesExpand
Optimization of fermentation condition favoring butanol production from glycerol by Clostridium pasteurianum DSM 525.
The initial fermentation conditions for butanol production from pure glycerol by Clostridium pasteurianum DSM 525 were optimized via a central composite design and a quadratic model was able to predict butanol yield as a function of all four investigated factors. Expand
Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum
This report has developed and exemplified a robust tool kit for the metabolic engineering of C. pasteurianum and used the system to make the first reported in-frame deletion mutants of pivotal genes involved in solvent production, namely hydA (hydrogenase), rex (Redox response regulator) and dhaBCE (glycerol dehydratase). Expand
Improved n-butanol production by a non-acetone producing Clostridium pasteurianum DSMZ 525 in mixed substrate fermentation
The results demonstrated that C. pasteurianum DSMZ 525 is well suitable for n-butanol production from mixed substrates of biomass hydrolysate and glycerol and represents an alternative promising production strain. Expand
Influence of nutritional and operational parameters on the production of butanol or 1,3-propanediol from glycerol by a mutant Clostridium pasteurianum.
It was found that N2 sparging is a suitable strategy to maximize the titer, yield and productivity of 1,3-PDO using C. pasteurianum. Expand
Effective isopropanol–butanol (IB) fermentation with high butanol content using a newly isolated Clostridium sp. A1424
BackgroundAcetone–butanol–ethanol fermentation has been studied for butanol production. Alternatively, to achieve acetone-free butanol production, use of clostridium strains producing butanol andExpand
The role of 1,3-propanediol production in fermentation of glycerol by Clostridium pasteurianum.
This study shows that as process pH decreased, the rate of cell growth and CO2 production also decreased, resulting in slower fermentations, increased duration of butanol production and higher butanol concentrations and yields. Expand


Fermentative butanol production by clostridia
This article reviews biotechnological production of butanol by clostridia and some relevant fermentation and downstream processes and the strategies for strain improvement by metabolic engineering and further requirements to make fermentative butanol production a successful industrial process. Expand
Butanol production from renewable biomass: Rediscovery of metabolic pathways and metabolic engineering
Recent discoveries of metabolic pathways for butanol production and the metabolic engineering strategies being developed are reviewed. Expand
Fermentation of glycerol by Clostridium pasteurianum — batch and continuous culture studies
  • H. Biebl
  • Medicine, Chemistry
  • Journal of Industrial Microbiology and Biotechnology
  • 2001
The fermentation of glycerol by Clostridium pasteurianum was studied with respect to product formation as influenced by the culture conditions, and it appears that the regulation of the numerous fermentation pathways occurring in this organism is not very strict. Expand
Production of acetone, butanol and ethanol by Clostridium beijerinckii BA101 and in situ recovery by gas stripping
We examined the effect of gas-stripping on the in situ removal of acetone, butanol, and ethanol (ABE) from batch reactor fermentation broth. The mutant strain (Clostridium beijerinckii BA101) was notExpand
3-Methyl-1-butanol production in Escherichia coli: random mutagenesis and two-phase fermentation
A mutagenesis approach in developing a strain of Escherichia coli for the production of 3-methyl-1-butanol by leveraging selective pressure toward l-leucine biosynthesis and screening for increased alcohol production is demonstrated. Expand
Production of propionic acid from whey permeate by sequential fermentation, ultrafiltration, and cell recycling
This article deals with the production by fermentation of a mycostatic and aromatic food additive based on propionic acid. Membrane bioreactors have been used from laboratory scale up to pilot andExpand
Butanol, ‘a superior biofuel’ production from agricultural residues (renewable biomass): recent progress in technology†
This article reviews bioconversion of plant materials such as wheat straw (WS), corn stover (CS), barley straw (BS), and switchgrass (SG) to butanol and process technology that converts theseExpand
Effect of cell recycle on continuous butanol-acetone fermentation with Clostridium acetobutylicum under phosphate limitation
SummaryAn overflow filtration unit for cell recycle with Clostridium acetobutylicum was developed. A cellulose-triacetate ultrafiltration membrane with a cut-off volume of 20 000 MW was found to workExpand
Glycerol: a promising and abundant carbon source for industrial microbiology.
Glycerol bioconversion in valuable chemicals, such as 1,3-propanediol, dihydroxyacetone, ethanol, succinate etc. is discussed in this review article. Expand
Metabolic engineering of Clostridium acetobutylicum M5 for highly selective butanol production.
A higher level of acetate accumulation was observed during fermentation of the M5 strain complemented with adhE1 and/or ctfAB, a plausible reason for this phenomenon is that the cellular metabolism was shifted towards acetate production to compensate reduced ATP production during the largely growth-associated butanol formation by the M 5(pIMP1E1AB) strain. Expand