Continued in vitro growth of fibroblast-like cells (RBCF-1) derived from the caudal fin of the fish, Carassius auratus

@article{Shima1980ContinuedIV,
  title={Continued in vitro growth of fibroblast-like cells (RBCF-1) derived from the caudal fin of the fish, Carassius auratus},
  author={Akihiro Shima and O Mizushima Works Nikaido and Shigeo Shinohara and Nobuo Egami},
  journal={Experimental Gerontology},
  year={1980},
  volume={15},
  pages={305-314}
}
Abstract Fibroblast-like cells derived from primary culture of the caudal fin of a 2-year old goldfish were used to find out whether or not fish cells have a limited in vitro lifespan during the observation period of 22 months. The cells cultivated at 37°C (RBCF-1 line) attained exponential growth from the beginning of culture and reached to 200 population doubling number ( PDN ) at the 310th day. The growth rate of the cells cultured at 30°C (RBCF-2 line) was almost the same as that of RBCF-1… CONTINUE READING
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