Continued DNA synthesis in replication checkpoint mutants leads to fork collapse.

@article{Sabatinos2012ContinuedDS,
  title={Continued DNA synthesis in replication checkpoint mutants leads to fork collapse.},
  author={Sarah A. Sabatinos and Marc D. Green and Susan L. Forsburg},
  journal={Molecular and cellular biology},
  year={2012},
  volume={32 24},
  pages={
          4986-97
        }
}
Hydroxyurea (HU) treatment activates the intra-S phase checkpoint proteins Cds1 and Mrc1 to prevent replication fork collapse. We found that prolonged DNA synthesis occurs in cds1Δ and mrc1Δ checkpoint mutants in the presence of HU and continues after release. This is coincident with increased DNA damage measured by phosphorylated histone H2A in whole cells during release. High-resolution live-cell imaging shows that mutants first accumulate extensive replication protein A (RPA) foci, followed… CONTINUE READING
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