Context-dependent contributions of backbone hydrogen bonding to β-sheet folding energetics

  title={Context-dependent contributions of backbone hydrogen bonding to β-sheet folding energetics},
  author={S. Deechongkit and H. Nguyen and E. Powers and P. Dawson and M. Gruebele and J. W. Kelly},
  • S. Deechongkit, H. Nguyen, +3 authors J. W. Kelly
  • Published 2004
  • Chemistry, Medicine
  • Nature
  • Backbone hydrogen bonds (H-bonds) are prominent features of protein structures; however, their role in protein folding remains controversial because they cannot be selectively perturbed by traditional methods of protein mutagenesis. Here we have assessed the contribution of backbone H-bonds to the folding kinetics and thermodynamics of the PIN WW domain, a small β-sheet protein, by individually replacing its backbone amides with esters. Amide-to-ester mutations site-specifically perturb… CONTINUE READING
    231 Citations
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