Context-dependent contributions of backbone hydrogen bonding to β-sheet folding energetics
@article{Deechongkit2004ContextdependentCO, title={Context-dependent contributions of backbone hydrogen bonding to β-sheet folding energetics}, author={S. Deechongkit and H. Nguyen and E. Powers and P. Dawson and M. Gruebele and J. W. Kelly}, journal={Nature}, year={2004}, volume={430}, pages={101-105} }
Backbone hydrogen bonds (H-bonds) are prominent features of protein structures; however, their role in protein folding remains controversial because they cannot be selectively perturbed by traditional methods of protein mutagenesis. Here we have assessed the contribution of backbone H-bonds to the folding kinetics and thermodynamics of the PIN WW domain, a small β-sheet protein, by individually replacing its backbone amides with esters. Amide-to-ester mutations site-specifically perturb… CONTINUE READING
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